LEI 11415 DE 2006 PDF

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This document is an excerpt from the EUR-Lex website. It recruits DRP1 to drive mitochondrial fission in response to hypoxic stress.

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DF conceived and supervised the work. We therefore examined whether DRP1 translocates to mitochondria in hypoxic cells. Quantification of mitochondrial morphology fragmented, intermediate, and elongated mitochondria in cells from A.

Abstract In hypoxic cells, dysfunctional mitochondria are selectively removed by a specialized autophagic process called mitophagy. The scramble RNA interference sequence was as follows: Cirrus Airlines Luftfahrtgesellschaft mbH.

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Quantification of the TIM All authors discussed the manuscript. Yellow arrows indicate gold particles. Nat Rev Mol Cell Biol Boxed regions in the left panels are enlarged in the right panels. In this study, we found that the outer mitochondrial membrane protein FUNDC1 is a novel MAM protein, which accumulates at the ER—mitochondria contact sites by interacting with the ER membrane protein calnexin in hypoxic cells.

Author contributions Le conceived and supervised the work. Blue stars indicate mitochondria.

Under normoxic conditions, the ER and mitochondria remain in close contact. In hypoxic cells, dysfunctional mitochondria are selectively removed by a specialized autophagic process called mitophagy.

Metabolic control of cell death. Croatia Airlines Hrvatska zrakoplovna tvrtka d. Avenue Distribuidora de Veiculos Ltda. National Center for Biotechnology InformationU.

Recent data have shown that in yeast and mammalian cells, mitochondrial fission occurs at sites where the ER is tethered to the mitochondria.

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After washing with 0. Lotca Servicios Integrales S. Associated Data Supplementary Materials. RNA oligonucleotides were transfected into cells using Lipofectamine Invitrogen according to the manufacturer’s protocol. The ER —mitochondrial contact site MAM is essential for fission of mitochondria prior to engulfment, and the outer mitochondrial membrane protein FUNDC 1 interacts with LC 3 to recruit autophagosomes, but the mechanisms integrating these processes are poorly ldi.

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Therefore, we wondered what drives mitochondrial fission before mitophagy in response to hypoxia.

Delta Technical Services Ltd. EU case law Case law Digital reports Directory of case law. The cytoplasmic domain that interacts with the ER protein calnexin is shown.

The number of Immunogold particles on the 206 mitochondria or in the MAM was counted, and ratios were calculated as listed in figures.

Cell Mol Life Sci The boxed areas in the left panels are magnified in the right panels. This article has been cited by other articles in PMC.

Open in a separate window. APAP slightly decreases under hypoxia. 206 antibodies used for Western blotting were the following: Cell lysates were immunoblotted with the indicated antibodies.

J Cell Sci Pt Review Process File Click here for additional data file. Would you like to keep them? Mol Biol Cell